Sperm are inclined to extreme reactive oxygen species. Spermine and spermidine are secreted in massive quantities by the prostate and potent pure free radical scavengers and shield cells in opposition to redox dysfunction. Thus, we used boar sperm as a mannequin to review the polyamines uptake and elucidate whether or not polyamines protected sperm from reactive oxygen species stress. Seven mature and fertile Duroc boars (aged 15 to 30 months) had been used on this research.
In experiment 1, spermine and spermidine (3.6 ± 0.3 and three.3 ± 0.2 mmol/L, respectively) had been considerable in seminal plasma, and the content material of polyamine decreased after preservation at 17 °C for 7 days or incubation at 37 °C for six hours. In experiment 2, utilizing labeling of spermine or spermidine by conjugation with fluorescein isothiocyanate and ultra-high-performance liquid chromatography, we discovered that the buildup of spermine or spermidine in sperm was inhibited by quinidine and DL-tetrahydropalmatine (THP, natural cation transporters [OCT] inhibitors), however not mildronate and L-carnitine (natural cation/carnitine transporter [OCTN] inhibitors). In experiment 3, the addition of spermine or spermidine (0.5 mmol/L) within the extender resulted in larger motility, plasma membrane and acrosome integrity, and decrease reactive oxygen species stage after preservation in vitro at 17 °C for 7 days. In experiment 4, within the situation of oxidative stress (therapy with H2O2 at 37 °C for two hours), the addition of spermine (1 mmol/L) or spermidine (0.5 mmol/L) in extender elevated actions of glutathione peroxidase, glutathione reductase, and glutathione S-transferase; lowered glutathione and oxidized glutathione ratio; and alleviate oxidative stress-induced lipid peroxidation, DNA harm, mitochondrial membrane potential (ΔΨm) decline, adenosine triphosphate depletion, and intracellular calcium focus ([Ca2+]i) overload, thereby bettering boar sperm motility, the integrity of plasma membrane and acrosome in vitro.
These knowledge recommend that spermine and spermidine alleviate oxidative stress through the antioxidant capability, thereby bettering the efficacy of boar semen preservation.
Li R, Wu X, Zhu Z, Lv Y, Zheng Y, Lu H, Zhou Ok, Wu D, Zeng W, Dong W, Zhang T, Polyamines shield boar sperm from oxidative stress in vitro, Journal of Animal Science. 2022; 100(4): skac069. https://doi.org/10.1093/jas/skac069